Parvovirus B19 IHC with nuclear and cytoplasmic staining of infected giant pronormoblasts
Antibodies[]
Anti-parvovirus antibody targets the capsid proteins VP1 and VP2 on human parvovirus
Technical Info[]
Pretreatment with EDTA, incubation time of 30-60 minutes at room temp or 16-32 minutes at 37 degree Celsius.
Staining Pattern[]
Nuclear and cytoplasmic staining of infected pronormoblasts in bone marrow (see photo at right) or in placental tissues.
Pertinent Negatives[]
This antigen is typically NOT expressed by non-infected cells.
Practical Uses[]
Useful for evaluation in immunosuppressed individuals suspected of harboring Parvovirus infection but unable to produce IgG or IgM antibodies for serologic testing. PCR testing may also be performed in this situation.
Can also be used to evaluate placental tissue in cases of spontaneous abortion, especially if maternal blood is not available for serological testing.
References[]
Cell Marque Immunohistochemistry Reference Guide, Vol. 9, p. 170